Author(s): Iqra Nazir, Mohd Danish, Sauliheen Qadri and Tabinda Shabir Yaswi

Abstract: In the current study, the immunostimulatory effects of chitosan, vitamin C, Curcuma longa, Tinospora cordifolia, Withania somnifera, and Allium sativum on Cyprinus carpio haematopterus fingerlings were assessed. A total of 25 fish per treatment group (T₀, T₁, T₂, and T₃) were randomly assigned to receive fingerlings weighing 30.0±2 g each in duplicate. A combination of rice bran, deoiled mustard oil cake, deoiled soybean cake, and vitamin-mineral mixture was used to create four isoproteinous experimental diets. The phytobiotics, Vitamin C and Chitosan were incorporated into diet. Per kilogramme of feed, diet D1 included 2.5 g each of garlic, giloy, turmeric, and ashwagandha whereas the diet D₂ had control feed + (2.5 g of each plant) + 25 mg Vitamin C per kg feed. For every kilogram of feed, diet D3 included 2.5 grams of each plant and 2.5 grams of chitosan. T₀ group fishes were fed with D₀ diet, T₁ with D₁, T₂ with D₂ and T₃ with D₃ @ 5% body weight per day for 122 days. All treatment groups fed the experimental meal showed a significant (p<0.05) increase in the levels of total blood protein, albumin, globulin, A/G ratio, and ALT between the pre-challenged and post-challenged groups. The findings show that the tested phytobiotics, in combination with chitosan and vitamin C, have excellent immunostimulatory effects and have no negative effects on the health of Cyprinus carpio haematopterus in a culture system.

DOI: 10.33545/26174693.2024.v8.i3Sf.793