Vol. 9, Special Issue 9, Part Y (2025)

A laboratory experiment was carried out at the Food Quality Testing Laboratory, N.M. College of Agriculture, Navsari Agricultural University, Navsari during of year 2023-24. In this study 26 bacterial culture previously isolated from pyroxasulfone applied soil at food quality testing laboratory were used for determination of degradation potential of isolated microbes, they were separately inoculated in BHM broth supplemented with pyroxasulfone as sole carbon source and incubated at room temperature at different time interval. Pyroxasulfone was extracted using acetonitrile and analyzed by GC-MS/MS. The maximum degradation of pyroxasulfone on BHM medium was recorded in HP 1 culture on 5^th,10^th and 15^th day after inoculation which was followed by culture HP 7. Among 26 bacterial cultures HP 1 and HP 7 showed that the highest degradation of pyroxasulfone as compared to others. These two cultures viz., HP 1 and HP 7 were identified by 16S rRNA gene sequencing. Based on sequence homology, the culture HP 1 and HP 7 were identified as Streptomyces sp. HP 1 and Staphylococcus sp. HP 7.