Vol. 9, Special Issue 8, Part N (2025)

CRISPR/Cas genome editing has revolutionized plant biotechnology by enabling precise manipulation of plant genomes. In vegetable crops, CRISPR-based methods have been deployed to enhance resistance against biotic stresses (pathogenic fungi, bacteria, viruses, nematodes, and insects) and abiotic stresses (drought, salinity, heat, cold, flooding, heavy metals). This review provides a comprehensive overview of CRISPR/Cas mechanisms in plants and surveys major targets and outcomes in a wide range of vegetables, including tomato, potato, pepper, eggplant, cucumber, cabbage, lettuce, spinach, carrot, onion, garlic, beans, and peas. We discuss key susceptibility genes that have been knocked out or modified to confer resistance in each crop group, and we highlight examples of base editing, prime editing, multiplex editing, and promoter editing as advanced CRISPR applications. This detailed technical review aims to inform researchers of current progress and future opportunities in applying CRISPR/Cas to improve disease and stress tolerance in vegetables.