Vol. 9, Special Issue 7, Part I (2025)

A total of 120 Rhode Island Red laying hens were randomly selected. They were divided into 5 groups of 24 each with three replicates of 8 in each group in a Completely Randomized Design. The included treatments were (i) T₁ (Control group) diet was supplemented without antibiotics and bacteriophages, (ii) T₂ group the diet was supplemented with antibiotic (Oxytetracycline @ 50g/q of feed) and (iii) T₃, T₄ and T₅, bacteriophage was supplemented at different concentrations @ 0.20ml/litre of drinking water, @ 0.25ml/litre of drinking water, @ 0.30ml/litre of drinking water, respectively. Among the serum biochemical parameters in laying hens, concentrations of albumin, globulin, total cholesterol, triglycerides, phosphorus, and only total protein and calcium were significantly affected by bacteriophage supplementation. These shifts in biochemical markers suggest that bacteriophages influence key aspects of metabolism and nutrient balance. This study’s findings indicate that adding bacteriophage to the diet of laying hens measurably modulates their serum profile. These outcomes support the conclusion that bacteriophage inclusion could serve as a viable alternative to antibiotic use. Overall, the results suggest that bacteriophage-enriched diets can positively influence both biochemical health and production performance in laying hens.