Vol. 9, Special Issue 7, Part B (2025)

Cattle, sheep, and goats play crucial roles in the Indian agricultural sector, facing significant threats from Capripoxviruse (CaPV) such as lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV). These viruses are notable for their economic impact and the challenges they pose to international trade due to their indistinguishable serological profiles. The molecular differentiation of SPPV, GTPV, and LSDV has been hampered by limited data on genetic characterization. This study addresses this gap by employing in-silico restriction enzyme (RE) digestion analysis on key viral genes, EEV maturation protein, virion protein, RNA polymerase subunit, and EEV glycoprotein. The results reveal distinct RE patterns that effectively discriminate between the three viruses, offering a precise and reliable method for their identification. This approach not only enhances diagnostic accuracy but also supports robust epidemiological studies and the implementation of targeted control measures. By providing a valuable tool for molecular surveillance, this study contributes to better management and prevention of CaPV outbreaks, thereby safeguarding agricultural productivity and international trade.