Vol. 9, Special Issue 6, Part G (2025)

Seasonal variations in temperature, humidity, and radiation were identified as possible risks to the development and productivity of all domestic animals. The purpose of the current study was to determine the immunological response, hormonal profile, antioxidant enzyme activities and biochemical profile of Mandya sheep following antioxidant treatment over various seasons. The Livestock Research and Information Centre (Sheep), KVAFSU, Nagamangala, Mandya District, was the site of the study. During the study period, twenty-four female sheep between the ages of three and five who appeared healthy were chosen and fed separately. Group I (control), Group II (Vitamin E @ 250 mg /animal /day + Vitamin C @ 2 g /animal /day), Group III (Selenium @ 0.1 mg /animal /day + Zinc @ 150 ppm /animal /day), and Group IV (Vitamin E @ 250 mg /animal /day + Zinc @ 150 ppm /animal /day) were the four equal groups containing six animals each. Twenty days of supplementation were conducted throughout three distinct seasons: the summer (16 April 2018 to May 5th, 2018), the rainy (30 July 2018 to August 18th, 2018), and the winter (30 December 2018 to 18th January, 2019). Using the temperature and relative humidity meteorological data, the research period's temperature relative humidity index was computed. Once every season, following the twentieth day of supplementation, blood samples were taken. The current study's findings showed that there is Elevated glucose levels in the summertime suggest impaired glucose utilization in animals under stress. Antioxidant supplements alleviate heat stress, increase insulin release, and improve glucose utilization by body cells over time. Summertime decreases in triglycerides and other changes in the lipid profile suggested overuse, while supplemented groups showed higher levels. Dehydration throughout the summer caused higher levels of total serum protein, albumin, and urea; however, supplemented groups showed a decrease in urea levels, suggesting the protective effects of antioxidants.