Vol. 9, Special Issue 6, Part B (2025)

Airborne microbial contamination is a critical factor in the transmission of infectious diseases in both clinical and non-clinical environments. Monitoring microbial air quality is essential for ensuring biosafety and public health, particularly in environments with dense human or animal populations. This study aims to evaluate and compare two air sampling methods-the Settle Plate Method and the Slit Sampler Method-across four distinct environmental settings: a veterinary hospital, an animal farm, a classroom, and a microbiology laboratory. A total of 96 samples were collected (12 samples per method per location), and microbial loads were assessed in terms of Bacteria Carrying Particles (BCPs). The slit sampler method consistently recorded significantly higher bacterial counts than the passive settle plate method across all locations. The highest microbial load was found in the animal farm, while the laboratory had the lowest. These findings underscore the importance of method selection in environmental microbiological surveillance and advocate for active air sampling, especially in high-risk zones.