Vol. 9, Special Issue 4, Part F (2025)

An experiment was aimed to study the effect of supplementation of synbiotic on immune and antioxidant response in broiler chicken. Two hundred and fifty (250) day old broiler chicks were randomly allotted to five treatments with 10 replicates of five birds in each. The treatments consist of a Basal diet based on corn-soybean without antibiotic as negative control (NC), with antibiotic growth promoter (Lincomycin @100 g/MT) as positive control (PC), three experimental diets consist of probiotic (Bacillus subtilis, B. coagulans, B. licheniformis and Saccharomyces boulardii) @ 200 g/MT, prebiotic (MOS & β-glucans) @ 500 g/MT and synbiotic (Bacillus subtilis, B. coagulans, B. licheniformis and Saccharomyces boulardii, MOS & β-glucans) @ 200 g/MT. The feed and water were offered ad libitum. The results revealed that Supplementation of probiotic, prebiotic and synbiotic did not influence (p>0.05) the phytohemagglutinin-P (PHA-P) and humoral immune response to ND vaccine at 6^th week of age. Significantly (p<0.05) higher Glutathione Reductase (GSHRx) concentration was observed in probiotic group compared to the control, prebiotic and AGP diets. Whereas, GSHRx concentration was significantly (p<0.05) higher in prebiotic and synbiotic groups compared to AGP group. There was no significant (p>0.05) difference among synbiotic, control and prebiotic groups. The concentration of Glutathione Peroxidase (GSHPx) was significantly (p<0.05) higher in prebiotic compared with control and AGP. Whereas, there was no significant (p>0.05) difference among prebiotic, probiotic and synbiotic groups. However, significantly (p<0.05) higher GSHPx concentration was observed in probiotic compared to AGP groups. There was no significant (p>0.05) difference in the superoxide dismutase concentration in the serum among all the dietary treatments.