Vol. 9, Special Issue 3, Part G (2025)

Current research attempts to determine minerals and electrolytes in milk might be utilized for diagnosing subclinical mastitis in Murrah buffaloes. In mid-lactation, 24 healthy Murrah buffaloes from Livestock Farm at College of Veterinary Science and Animal Husbandry in Mhow, in addition to those from neighbouring villages, became subjects of research investigation. Group I consisted of bacteriologically negative samples with SCC ≤ 200,000 cells/ml milk (CMT-negative), Group-II of bacteria samples with SCC ≥ 200,000 ≤ 500,000 cells/ml milk (CMT-positive), and Group-III of bacteria samples with SCC ≥ 200,000 ≤ 500,000 cells/ml milk (CMT-positive) depending on udder health conditions. Positive CMT test One animal's quarter samples have been combined to generate single sample for somatic cell count (SCC). There had been significant (p<0.05) variations in average calcium and phosphorus levels between groups I, II, and III. Group III's mean sodium measurements have been determined to differ significantly (p<0.05) from those of groups I and II. However, there hasn't been a significant difference between group I and II. There was significant (p<0.05) difference in milk potassium between groups II, I, and III. There is significant (p<0.05) difference in milk chloride between groups III and I and II. It is concluded that subclinical mastitis induced marked change in level of milk minerals (calcium and phosphorus) and electrolytes (sodium, potassium and chloride) that could be employed for diagnosis of subclinical mastitis.