Vol. 9, Special Issue 3, Part F (2025)

The present investigations on “Effect of Probiotic Supplementation on Eggs Production Performance in Laying Hens” were conducted at the Poultry Unit, Division of Animal Husbandry and Dairy Science, College of Agriculture, Pune. A total number of 160 White leghorn laying hen, of 32 weeks of age at the beginning of the study were used for the present study. The laying hens were weighed and distributed randomly into four treatment groups viz., T₀ (Diet without Probiotic (Bacillus subtilis) (Control), T₁ (Diet + 500 gm Probiotic (Bacillus subtilis)/ton of feed.), T₂ (Diet + 1000 gm Probiotic (Bacillus subtilis)/ton of feed) and T₃ (Diet + 1500 gm Probiotic (Bacillus subtilis)/ton of feed.) with 40 laying hen in each treatment as replicates on equal weight basis. The laying hens were fed the same experimental diets with different levels of probiotic supplementation in laying hen trial during the experimental period from 32 to 41 weeks of age. The objectives of this study to evaluate the egg production performance of White Leghorn laying hens supplemented with Bacillus subtilis and to assess the feed conversion ratio to determine the efficiency of feed utilization. Key performance indicators, including feed intake, hen day egg production (HDEP), egg mass and feed conversion ratio (FCR) were assessed over the experimental period. Results indicated that hens receiving Bacillus subtilis supplementation (especially T₂) exhibited significantly higher feed intake and enhanced egg production, with HDEP values of 75.96, 79.25, 82.42 and 77.58 percent for T₀, T₁, T₂ and T₃, respectively. The overall egg mass also increased notably in the T₂ group, reaching an average of 45.31 g compared to 40.62 g in the control group. Furthermore, the lowest FCR was recorded in the T₂ group at 1.46, demonstrating improved feed efficiency in converting feed to egg mass. In conclusion, the incorporation of Bacillus subtilis in poultry diets may provide a viable approach to improve the overall productivity and specifically, the supplementation resulted in an improvement in egg weight an increase in egg mass was observed in the T₂ treatment group.