Vol. 9, Special Issue 2, Part I (2025)

The present study was conducted to evaluate the effect of different levels of tulsi leaf powder (TLP) and prebiotic mannan oligosaccharide (MOS) as non-nutrient feed additives on biochemical parameters of Japanese quail (Coturnix coturnix japonica). A total of three hundred and seventy-eight adult Japanese quail layers were individually weighed to 0.1 g accuracy and 54 adult birds each were randomly allotted to seven different treatments. Each treatment had three replicates of 12 females and 6 males and maintained with 2:1 sex ratio. The experimental diets were control (T₁), TLP @ 0.5% (T₂), TLP @ 1.0% (T₃), TLP @ 1.5% (T₄), MOS @ 0.5% (T₅), MOS @ 1.0% (T₆) and MOS @ 1.5%. At the end of 42 days experiment period, the birds were slaughtered and blood samples were collected for determination of serum biochemical parameters viz., total protein, cholesterol and triglycerides. The serum total protein content of 12.08 g/dl is significantly (p<0.05) higher in T₅ group compared to control and other treatment groups. The results also revealed that significantly (p<0.05) lower serum cholesterol (20.67 mg/dl) observed in T₃ group supplemented with TLP at 1.00 percent level compared to control group. Among all treatment groups serum triglycerides level (mg/dl) were significantly lower (p<0.05) in T₇ group fed with MOS @ 1.5% level compared to control group. It was concluded that supplementation of different levels of TLP and prebiotic MOS as non-nutrient feed additives significantly improved the biochemical profiles of Japanese quails.