Vol. 9, Special Issue 2, Part H (2025)

This study was to compare the seminal plasma proteins profiles of Gir bull and Murrah buffalo bull to examine the reproductive performance in terms of seminal plasma polypeptide properties as well as semen concentration, Mass and Individual motility and shape of spermatozoa. Purification and immuno-biochemical characterization of fertility associated proteins in Murrah and Gir bulls are major immuno-dominant proteins that have a significant impact in fertility. Seminal plasma was collected from evidently normal semen comprising viable sperm for extraction. The Murrah buffalo and Gir cattle herds contribute the most to India's milk production, hence it is important to comprehend the proteomics of Gir bull seminal plasma proteins and to compare them to those of buffalo bulls in order to better understand their molecular characteristics. Therefore, this research compares and contrasts our existing understanding of the expanding field.