Vol. 9, Special Issue 2, Part G (2025)

Poultry is one of the fastest growing segments of the agricultural sector in India.

The poultry sector in India has shown impressive growth, with poultry meat production increasing at an average annual rate of 80% between 2014 – 15 and 2021-22. The continuous growth of this industry had resulted in massive quantities of solid waste as feathers, viscera, bones, and dead on arrival. Under-utilization of these byproducts not only leads to loss of potential revenues but also to the added and increasing cost of disposal. Chicken intestine as a poultry industry byproduct is rich in protein, contains various endogenous enzymes and can be prepared as a hydrolysate via autolysis. Chemical, enzymatic and microbial methods can produce hydrolysed peptides from chicken intestines. In this study, enzymatic hydrolysis by using protease P (phycomycetes) fungal origin enzyme followed by ultrafiltration of chicken intestines proved to be more ecofriendly in the extraction of bioactive peptides.

The characteristics of the extracted bio active peptides were analysed by SDS PAGE and their bioactivity was assessed by DPPH and alpha amylase inhibition assays. The extracted bioactive peptides were dried in to powder and incorporated in canine pet food at levels of 5%, 6% and 7% of bioactive peptides. Hence, the study had opened up a way for the better utilization of chicken intestine in to bioactive peptides with multi-functional properties which in turn could be utilized in pet industry, which otherwise would have remained a waste posing a direct impact on the economy and environmental pollution.