Vol. 9, Special Issue 1, Part J (2025)

Tick-born intracellular blood parasites, T. annulata are a serious economic constraint to the livestock industry. In the present study, a total of 200 blood samples were collected from different locations belonging to the Mathura and Agra districts of Uttar Pradesh. The samples were further subjected to microscopic, and molecular. In microscopic examination, 11% prevalence of T. annulata was observed. The molecular test based on Cyt b gene showed prevalence of 28% for T. annulata. Under the study, various clinical signs related to the disease were also considered and fever was observed in higher frequency in tick-borne diseases under the study. In the epidemiological study, there was a significant (p<0.05) difference observed in the seasonal occurrence of parasites; however, no significant difference was observed in between other criteria like age, sex, breed and geographical area. The present epidemiological investigation can be helpful in the accurate diagnosis of T. annulata. However, further study on standardization and validation at a large sample size is further required.