Vol. 9, Special Issue 1, Part J (2025)
Isolation and characterization of endophytic bacteria from tomato (Solanum lycopersicum) plant
Endira Kujur, Diptimayee Dash and Balram Sahu
The study was conducted at Indira Gandhi Krishi Vishwavidyalaya, Raipur, during 2022-2023 with the objective to evaluate endophytic bacteria of tomato plants. A total of 24 endophytic bacterial isolates (ESR1, EBR2, EAR4 ERR5, EAS12 EJS11 ERS13, ESL17, EBL18, ERL21, and EML24) were obtained from different plant tissues including root (R), stem (S) and fresh leaves (L) regions of tomato plants collected from several locations in Chhattisgarh. This study involves morphological and biochemical characterization of isolated bacterial endophytes. Among twenty-four isolates, eighteen isolates were gram positive, eleven isolates were found positive to siderophore production, nine isolates were found for phosphate solubilization while HCN production was not observed. Siderophore test was observed more prominent in EBR2, ERR5 and EJS11 isolates. Significant quantity of IAA produced by these isolates in-vitro after 24 hours of incubation with added precursor Tryptophan ranging from 8.80μg ml-1 to 18.00μg ml-1 being significantly maximum in EAR4 followed by ERR5, EAS12 isolates.
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