Vol. 9, Special Issue 12, Part P (2025)

Using the rumen fluid of adult Surti buffaloes, an in-vitro rumen fermentation study was carried out to assess the impact of adding Solid State Fermented Biomass (SSF) biomass to the total mixed ration (TMR) on methanogenesis, dry matter digestibility, and total gas generation. For in-vitro gas generation experiments, SSF biomass was supplemented with TMR (65% wheat straw and 35% concentrate) at 0, 1, 2, 3, 4, 5, 6, 7, and 8%. At a 3% level of SSF biomass addition in TMR, the in-vitro study's results showed considerably (p<0.05) higher IVDMD (58.43%) and decreased CH4 generation (3.58 ml CH4/100 mg DDM). The most appropriate level of SSF biomass supplementation for additional in-vivo research in adult Surti buffaloes was determined to be 3% based on the overall findings of in-vitro experiments.