Vol. 9, Special Issue 12, Part N (2025)

Chemical nanocompounds (CNCs) are increasingly being utilized in a variety of fields, such as cosmetics, pharmaceuticals, and medicine, yet their precise effects on biological systems remain insufficiently understood and warrant further investigation. In this study, CNCs were administered intratracheally to rats, and subsequent blood samples were meticulously analyzed using advanced analytical techniques and multiplex assays. These methods were employed to comprehensively assess the impact of CNCs on various inflammatory biomarkers and clinical enzyme levels, which are essential for evaluating exposure and conducting risk assessments in biological contexts. During the analysis, significant dose-dependent increases in several inflammatory biomarkers, specific enzymes, and haptoglobin levels were observed, indicating a pronounced biological response to the CNC exposure. This investigation significantly contributes to a deeper understanding of the biological effects associated with CNCs and underscores the utility of advanced analytical techniques in detecting chemical stress within biological systems.