Vol. 9, Special Issue 10, Part Q (2025)
Synergistic effects of biological agents on the biohardening of in vitro-raised Spathiphyllum plantlets
M Clement Kanth, K Ravindra Kumar, AVD Dorajee Rao and A Snehalatha Rani
Spathiphyllum is a popular and widely cultivated indoor ornamental plant, valued across the world for its aesthetic appeal and adaptability to indoor environments. The efficiency of in vitro multiplication in Spathiphyllum is constrained by low survival rates and slow plantlet growth. Hence, the current study was under taken to study the influence of microbial inoculants on survival, growth, physiological traits and disease dynamics of micro-propagated Spathiphyllum plantlets during acclimatization. The present investigation was carried out at Regional Horticultural Research Station, Kovvur, East Godavari District of Andhra Pradesh during the year 2024-25. The experiment was conducted with different bioagent combinations viz., B1 (Trichoderma reesei + AMF), B2 (Trichoderma reesei + Azotobacter), B3 (Trichoderma reesei + PSB), B4 (Pseudomonas fluorescens + AMF), B5 (Pseudomonas fluorescens + Azotobacter), B6 (Pseudomonas fluorescens + PSB), B7 (Trichoderma reesei + Pseudomonas fluorescens), and B8 (control). Among the treatments, significant increase in plant survival (92%), maximum plant height (8.17 cm), number of leaves (12.3), leaf area (32.5 cm²), chlorophyll content (SPAD 45.8), root length (5.92 cm) was recorded in potting media enriched with Pseudomonas fluorescens and Phosphate solubilizing bacteria. Higher root system development and significant increase in plant survival was also recorded in AMF in combination with Pseudomonas fluorescens and Trichoderma reesei.
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