Vol. 8, Special Issue 9, Part P (2024)

Isolation, identification and cellulase enzyme synthesis abilities of bacteria isolated from termite gut. The gram positive and the negative bacteria can be differentiated visually by this technique (Gram staining method), after isolation these isolates were confirmed to species level on phylogenetic model. The cellulolytic bacterial strains were also cultivated on a caborxy methylcellulose (CMC) medium to assess their cellulase activity with Gram's iodine staining for the formation of halos. A wider halo zone suggested a higher potential to degrade cellulose, and thus commercial usage of these bacteria in the degradation of biomass. Further research work was also done to investigate the synthesis of cellulase enzymes in different environmental conditions and emphasis were given for optimizing other parameters like nutrient composition, incubation period etc. This study emphasizes the biotechnological potential of termite gut bacteria in high-lignocellulolytic efficiency and production biofuels using plant biomass and is showing a new way for researchers describe characteristics, diversity taxonomic composition as well function to this complex most diverse symbiotic system.