Vol. 8, Special Issue 9, Part N (2024)

The biosynthesized metal oxide nanoparticles are of particular interest due to their wide applications in diverse fields. An experiment was conducted in 2023-24 in which, copper oxide nanoparticles were successfully synthesized using cell free extract of Trichoderma asperellum fungus. The biosynthesized nanoparticles were characterized by Ultraviolet–visible (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM) UV– visible spectrum of biosynthesized of Copper nanoparticles showed a peak at 575 nm. The Cu NPs synthesized were found to have an average particle size distribution of 156.16nm (78.08 r.nm). Fourier transformation infrared (FTIR) Graph shows 22 peak point which represent 18 functional groups. XRD analysis showed the face-centred cubic (fcc) structure of Cu NPs .SEM analysis cleared that spherical, cubes and tetrahedron structures with average particles size is 103.8 nm at a magnification of 200 nm.