Vol. 8, Special Issue 9, Part M (2024)

Antioxidant enzyme status and lipid peroxidation have significant effects on normal functions of spermatozoa including its morphological structure. Many antioxidative enzymes play a protective role in lipid peroxidation of sperm cell membrane depending upon the level of stress. In this study, the correlation of lipid peroxidation with antioxidative enzyme status and physico-morphological character has been evaluated in neat semen during the winter and summer seasons from five healthy Hariana breeding bulls. A total of 140 semen ejaculates (60 winter and 80 summer) of Hariana bulls were collected. The temperature humidity index (THI) was 68.41±0.67 during winter and 78.84±0.65 during summer season. For winter VS summer season, the overall mean value of ejaculated volume was 4.29±0.11 VS 3.72±0.10 ml, initial motility 83.66±0.38 VS 71.70±1.02%, sperm concentration 1441.51±48.94 VS 1540.14±50.48 million/ml, sperm abnormalities 3.34±0.12 VS 4.05±0.12%, mass motility 3.80±0.07 VS 2.53±0.10, sperm viability 84.06±0.61 VS 73.24±0.94, HOST reactive sperm 65.71±1.07 VS 63.57±0.83% and acrosomal integrity 81.89±0.81 VS 69.96±1.18% was significantly lower during summer as compared to winter season at (p<0.05) level. The antioxidant enzyme i.e catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase activity was significantly (p<0.05) higher during the winter season as compared to the summer season. The lipid peroxidation was significantly (24.70±0.36 VS 25.13±0.18 nmoles MDA/ml) lower in the winter season as compared to the summer season. Lipid peroxidation was positively correlated (p<0.05) with sperm abnormalities, ejaculate volume, and initial motility and negatively correlated (p<0.05) with sperm concentration, mass motility, sperm viability, HOST and acrosomal integrity. Seasonal variations may affect the relationship of antioxidant enzyme activity with lipid peroxidation production and physico-morphological characteristics of semen in Hariana bulls.