Vol. 8, Special Issue 9, Part M (2024)

Multiplex genome editing has emerged as a transformative approach in plant biotechnology, enabling precise and simultaneous modifications of multiple genetic loci within a single generation. This review explores the key genome editing tools, including Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), CRISPR-Cas9, and TATSI (Transcription Activator-like effector-mediated Targeted Single-base Insertion). Each tool's mechanisms, advantages, and limitations are discussed, highlighting their contributions to crop improvement. The application of these technologies has facilitated the development of crops with enhanced traits, such as increased yield, disease resistance, and improved nutritional content. By enabling targeted modifications in complex genomes, these tools are revolutionizing traditional breeding practices and offering new opportunities for sustainable agriculture. This review also addresses the challenges and future prospects of multiplex genome editing, emphasizing its potential to accelerate crop improvement and meet the growing demands of global food security.