Vol. 8, Special Issue 9, Part C (2024)

Abstract: This study evaluates the effectiveness of Steinernema abbasi PN-1 as a biological control agent against Achroia grisella larvae, a major pest in beekeeping. By testing a range of nematode concentrations from 25 to 600 infective juveniles per milliliter (IJs/ml), the research examines the relationship between nematode dose and larval mortality over a 54-hour period. The results demonstrate a clear dose-dependent response, with higher concentrations leading to increased mortality rates and shorter median lethal times (LT₅₀). Specifically, complete mortality (100%) was observed at concentrations of 600 IJs/ml and 400 IJs/ml within 48 hours, underscoring the nematode's rapid and potent control capabilities. Additionally, as nematode concentration increased, LT50 values significantly decreased, indicating faster larval mortality at higher doses. A table presenting the lethal concentrations (LC₅₀ and LC₉₅) and dose-response parameters for S. abbasi against A. grisella larvae underscores the nematode's effectiveness. The LC₅₀ was determined to be 34.14 IJs per insect, while the LC₉₅ was 904.99 IJs per insect, reflecting substantial control potential. These findings suggest that S. abbasi PN-1 could serve as a promising and environmentally sustainable alternative to chemical pesticides for managing A. grisella infestations in beekeeping. Future research should prioritize field trials and optimization of application strategies to enhance practical use.