Vol. 8, Special Issue 5, Part E (2024)

Abstract: Uterus of total sixty non descript buffaloes were studied during proestrus, estrus, metestrus, diestrus, anestrus and early pregnancy. Each group was consisted of ten samples. Histochemical reactions of Periodic Acid Schiff, PAS Alcian blue, Best Carmine, alkaline phosphatase, Acid phosphatase activity was demonstrated as per the standard protocol. Staining reactions of correlative changes in the carbohydrate and enzymes in the epithelium, lamina propria, uterine glands, myometrium and perimetrium of uterine horns were observed. The greatest secretory activity of uterine glands was observed during the diestrus. The reactions for presence of glycogen in the lamina propria, uterine glands and myometrium showed maximum reaction for glycogen material in the early pregnancy. The PAS-positive material in the uterine epithelium, lamina propria and uterine glands showed intense reactions during proestrus and estrus. It is hoped that this study would be helpful for better interpretation of changes caused in diseased conditions of the uterus in buffalo.