Vol. 8, Special Issue 5, Part A (2024)

Fractionation of rice seed storage proteins in two rice genotypes

Author(s):

Muttugoud Revataganv, HB Mahesh, B Manjunath, Jitendra Kumar and Harinikumar KM

Abstract:
This study investigated the fractionation of seed storage proteins in two rice genotypes, BPT-5204 and HPR-14, focusing on the estimation and characterization of albumin, globulin, glutelin, and prolamin fractions. Fractionation was based on solubility properties, with subsequent estimation of protein concentrations between the genotypes. The SDS-PAGE analysis further elucidated the various molecular weights for each protein fraction. Our results indicated that glutelin is the most abundant fraction in rice grains, followed by albumin, globulin, and prolamin. Despite employing different rice varieties, minimal differences were observed in protein banding patterns, highlighting the consistency in protein composition across genotypes. This comprehensive analysis enhanced our understanding of rice protein fractionation, putting the groundwork for future research on rice protein functionality and nutritional significance. The findings of this study contribute to the ongoing efforts to optimize rice protein utilization in various food and feed applications, potentially addressing global nutritional challenges.

Pages: 39-44  |  348 Views  149 Downloads

How to cite this article:
Muttugoud Revataganv, HB Mahesh, B Manjunath, Jitendra Kumar and Harinikumar KM. Fractionation of rice seed storage proteins in two rice genotypes. Int. J. Adv. Biochem. Res. 2024;8(5S):39-44. DOI: 10.33545/26174693.2024.v8.i5Sa.1116