Author(s): AS Kardani, LC Modi, NF Chaudhari, AA Pathan, HC Sharma and DB Bhoi

Abstract: The present research work was conducted to examine effect of different concentrations of Black cumin (Nigella sativa) seeds extract in Tris egg yolk citrate extender on sperm viability of cryopreserved Surti buck semen. Total 64 semen ejaculates were collected from four Surti buck (16 ejaculates/buck) twice a week by artificial vagina method. Semen samples were diluted with Tris egg yolk citrate extender and Black cumin (Nigella sativa) seeds aqueous extract was supplemented at different concentrations viz. 0% (T₁), 3% (T₂), 4% (T₃), 5% (T₄) (pH 6.5-6.8) maintaining final concentration of 100×10⁶ sperm/ml. After making the groups, the semen samples were examined for structural plasma membrane integrity (viability) (%) and functional plasma membrane integrity (HOST) (%) for all groups. Result showed that highest mean live sperm (%) and mean host reacted sperm (%) was found at initial, pre-freeze and post-thaw stage in T₃ group followed by T₂, T₄ and T₁ group. It was concluded that post-thawed viability and host reacted sperm (%) parameters were well maintained at 4% supplementation of Black cumin (Nigella sativa) seed extract in Tris egg yolk citrate extender.

DOI: 10.33545/26174693.2024.v8.i4Sc.943