Author(s): Preeti Verma, Anand Kumar Jain, Aditya Mishra, Deepika D Jesse, Sanju Mandal, Anil Gattani, Pragati Patel, Purnima Singh and Manish Jatav

Abstract: A study was carried out in order to investigate the effect of daily supplementation with Withania somnifera (Ashwagandha) roots in powder form on physiological, haematological, and hormonal indices in buffalo calves during heat stress. Twelve healthy Murrah buffalo calves were divided into control and supplemented groups were taken for the study. In supplementation to basal diet, Withania somnifera (25g/daily) was given at morning time from day 1 up to day 45. Throughout the experiment, both the control and supplemented groups had non-significant rectal temperatures at fortnightly intervals. Significant difference (p<0.05) was observed in pulse and respiration rate were observed at fortnightly interval in both control and supplemented groups. Blood glucose, protein, and cholesterol concentrations in the supplemented and control groups did not differ significantly (p>0.05) throughout the course of the study. Similarly, a non-significant difference (p>0.05) were observed in concentration of serum sodium, potassium and chloride in treatment groups as compared to control group. Non-significant difference (p>0.05) were observed in various haematological parameters, during the entire experimental period in both control and treatment group except RBC related parameters and platelets concentration. Non-significant difference (p≥0.05) were found in concentration of serum cortisol, T₃, T₄ and TSH were observed during the entire experimental period in both control and supplemented groups. In conclusion, Withania somnifera affects thyroid hormone response and cortisol release under summer stress. It lowers T₃, T₄, and cortisol levels. It lowers rectal temperature, increases respiration and pulse rate in animals, and helps them cope with the detrimental impacts of summer stress.

DOI: 10.33545/26174693.2024.v8.i4Sb.910