Vol. 8, Special Issue 4, Part B (2024)

Abstract: A study was carried out in dairy farms in and around Hyderabad. The buffaloes with the history of abnormal uterine discharge and repeat breeders that did not conceive in earlier services were selected through clinico-gynaecological examinations, estimation of pH of cervico-vaginal mucus and white side test of cervico vaginal mucus to diagnose the endometritis. A total of Thirty-two buffaloes with endometritis were selected and randomly divided into four groups of eight animals in each group. The buffaloes of group-I not given any treatment and were inseminated at observed estrus, Group II were administered intrauterine Lenovo I.U. 60ml intrauterine for 3 consecutive days from day of estrus, Group III were administered intrauterine E. coli lipopolysaccharide 100μg in 20ml of sterile phosphate buffer saline single dose on the day of estrus and Group IV were administered intrauterine Oyster glycogen 500mg in 30ml phosphate buffer saline single dose on the day of estrus and artificial insemination was done during subsequent observed estrus when white side test was found negative. According to the results it was concluded that the buffaloes with abnormal cervico-vaginal discharges and with conception failure could be effectively treated with the intrauterine infusion of E.coli LPS and Oyster glycogen to improve the fertility and conception rate. The E.coli LPS and Oyster glycogen can also be used effectively as immunomodulator with highest safety to the endometrium and without causing any systemic effects.