Author(s): Ramkaran, Sandeep Gera, Ravi Kumar, Pradeep Kumar and Sandeep Kumar

Abstract: The present study was conducted on 50 Hariana cattle to analyse association of HSP 27 gene with vital parameters and of milk production parameters. Genomic DNA was extracted from whole blood of a total of 50 lactating Hariana cattle. PCR amplification yielded products of 631bp for HSP 27 gene. Amplicons were subjected to direct sequencing and multiple sequence alignment using ClustalW revealed one nucleotide sequence variation in HSP 27 gene. In HSP 27 gene G225A was polymorphic with three genotypes AA, AB and BB in our resource population. Allele and genotype frequencies of HSP 27 gene were calculated using POPGENE software package. Genotype AA, BB and AB had frequencies 0.64, 0.28 and 0.08, respectively, whereas, allele frequency for A and B allele were 0.78 and 0.22, respectively. Effect of SNP variants on physiological parameters and milk parameters were analysed using least squares model with the help of SAS statistical software. Effect of genotypes on physiological function were found significant (p<0.05) for RR and HTC values. Least squares mean value for genotype AA, AB and BB were 67.85, 65.92 and 67.33 for PR, 101.40^oF, 101.46^oF and 101.11^oF for RT, 24.00, 22.10 and 27.19 for RR, 1.69, 1.75 and 2.11 for HTC respectively. Hariana cows with genotype AA had better thermotolerance than the other cows having genotypes AB and BB due to low HTC values. However, effect of genotypes on milk parameters was found to be non-significant.

DOI: 10.33545/26174693.2024.v8.i3Sf.795