Abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a potent dioxin congener, is recognised for its actions through the AhR pathway, leading to various detrimental effects in the body. Folic acid and vitamin B12, known as methyl donors in one-carbon metabolism, have recently been identified as AhR antagonists. In this study, the impact of folic acid and vitamin B12 on the viability of caprine granulosa cells exposed to varying levels of TCDD for 24 hours was investigated. The aim was to elucidate the potential role of these vitamins in mitigating the adverse effects of TCDD on cell viability. The results of the study revealed that supplementation with folic acid and vitamin B12 led to a reversal in the viability of TCDD-treated cells, although this effect did not reach statistical significance. The increase in the cell viability observed could have been mediated by the AhR antagonistic action of folic acid and vitamin B12 in TCDD-treated granulosa cells or due to the decreased production of reactive species as a result of supplementation of vitamin B12 and folic acid.
