Author(s): Surendra, Abhishek Gaurav, Sudesh Sharma, Ridhima Mahadeva, Manisha Doot and Vipin Bairwa

Abstract: To assess the genetic diversity of strains, genotypic characterization of foodborne bacteria such as Salmonella is very significant. Salmonella is a complex bacterial species with a wide number of serotypes and strains in different host populations, including animals, birds and humans. In total, 304 samples from poultry farms (water, feed, poultry faeces and carcass) and retail chicken meat sold in market were collected randomly from Udaipur district. Isolation of Salmonella strain from the samples collected from poultry farms and chicken meat sold in retail market was done according to IS 5887 (Part 3):1999. Isolation of DNA from pure culture of presumptive Salmonella isolates was done by using the Nucleo-pore^® gDNA fungal/bacterial mini kit (Genetix) as per the instruction mentioned in the kit manual. All the Salmonella isolates were screened for the presence of genus specific genes by using the PCR. Out of the 304 samples from poultry farms and retail chicken meat including water (n= 29), feed (n= 29), poultry feces (n= 106), carcass (n= 42), and retail chicken meat (n= 98), a total of four samples were found to be positive for Salmonella spp. giving a prevalence rate of 3.44%, 0%, 1.89%, 0% and 1.02% respectively. These 4 isolates were further confirmed through PCR by targeting thegenusspecificprimers(invAand16SrRNAgene)givingtheoverall prevalence to be 1.32% (4/304).

DOI: 10.33545/26174693.2024.v8.i1Sm.484