The study, conducted in 2021-2022 at AICRP on spices, Central Experiment Station, Wakavali, investigated the impact of spacing and biofertilizers on ginger (Zingiber officinale Rosc) cv Mahima using the pro tray seedling method. Spacing S3 consistently exhibited maximum growth and developmental characteristics, while S1 recorded the highest plant height. S3B3 emerged as the most effective combination, showing superior attributes in primary and secondary rhizomes and yield per plant. However, S1B3 excelled in yield per plot and yield per hectare. Biofertilizer treatment B3 consistently outperformed B4 in various growth parameters. The interaction between spacings and biofertilizer treatments emphasized S3B3 as the most favorable combination, except for plant height where S1B3 excelled. In conclusion, for higher yield per hectare, it is recommended to cultivate ginger with S1 spacing (30 x 20 cm) and B3 biofertilizer (Vesicular arbuscular mycorrhizae at a rate of 25 kg/h).