Author(s): Valeeta Marina Dsouza, Kirankumar Rotti, Satish M and Vinod Mavarkar

Abstract: Coleus forskohlii is a medicinal plant that can be used as a plant system to produce recombinant proteins due to its short duration of 6 to 7 months and ability to grow in moderate climates with light, medium, and drained soil. Callus induction and proliferation systems are useful for genetic improvement of this endangered multipurpose medicinal plant systems are a promising direction of protein production technology, providing a wide variety of bacterial to complex multi subunit glycosylated human proteins. Plant systems provide the stable inherited expression of a foreign gene by transforming the nuclear DNA and fast, highly effective production of the desired protein by extra chromosomal amplicons. They also provide an cost-effective and safe platform for vaccine development due to ease in scalability and a low risk of contamination with endotoxins or human pathogens. Coleus is suitable for transformation due to its abundant protein accumulation in leaves and easy propagation using cuttings. The standardization of regeneration protocol of Coleus forskohlii using leaf explants was conducted, using various concentrations of growth hormones such as auxin (IAA) and cytokinin (BAP). The results showed that the mean number of shoots per callus differed significantly between treatments, with the highest shoot formation being obtained in the combination of hormones containing 9mg L^-1 of BAP and 0.4 mg L^-1 of IAA. The callus texture obtained during this experiment was compact and fragile and the color of the callus was pale green to dark green. Significantly highest per-cent of callus, mean number of shoots per callus and the rooting was obtained in the combination containing 9mg L^-1 of BAP and 0.4 mg L^-1 of IAA with 94 percent, 11.16, and 6.34 respectively. Rooting and hardening were achieved with different concentrations of BAP and IAA after sub-culturing at the 60^th day.

DOI: 10.33545/26174693.2024.v8.i1Sd.310