Vol. 8, Special Issue 1, Part B (2024)

Abstract: The current investigation aimed to assess the serum total antioxidant capacity (TAC) of pubertal Marwari rams using an ethanolic dry extract of Moringa oleifera leaves (MOLEE). A total of twenty-four pubertal rams, aged 7-8 months and with an average body weight of 28 kg, were carefully chosen and randomly divided into four experimental groups, with six rams in each group. Group C received a standard diet without MOLEE supplementation, while groups T₁, T₂, and T₃ were provided with the same standard diet along with MOLEE at doses of 40, 80, and 160 mg/kg body weight respectively, for a duration of 90 days. Blood samples were collected every two weeks, and the serum was separated and stored at -20 °C for subsequent analysis of TAC using the ABTS decolorization assay. The findings revealed that the concentration of TAC (uM) was significantly (p<0.05) higher in groups T₃, T₂, and T₁ compared to group C. This study demonstrates that Moringa oleifera leaf extract can serve as a valuable feed supplement, enhancing sexual maturity and improving the overall antioxidant profile in pubertal Marwari rams.