Vol. 8, Special Issue 11, Part J (2024)

In this investigation total 21 rice cultures were screened for biotic and abiotic stress using 17 trait link markers. Among these cultures; RTN 17-1832-7-1-2-2-15 showed tolernace for salt and drought. The cultures RTN 1523-13-2-18 showed tolerance and resistance traits for salt and brown planthopper respectively. In abiotic stress, six cultures showed amplification towards specific Saltol linked allele. For drought tolerance, RTN 17-1832-7-1-2-2-15, RTN 1411-10-3-5-2-2-2-21, and RTN/MT-BR-201 showed amplification for Dr linked allele. In biotic stress, bacterial blight cultures Taichin, Thombe, and Black Rice-Chakav showed amplification for the Xa5 gene, and no amplification was shown for the Xa21 gene. For brown planthopper resistance was shown in cultures RTN 2021-SVIN097, RTN 1721-2-1-2-1-1-3, and RTN 1523-13-2-18 for the Bph25 gene and no amplification was shown for Bph26 gene. For gall midge four cultures showed amplification for Gm8 gene. The 17 primers showed an average 60.49 polymorphism. A total of 50 alleles were detected with an average of 2.94 alleles per primer. The polymorphism information content (PIC) values ranged from 0.090 to 0.789 with an average PIC value of 0.475 per primer. MVSP software was used to determine genetic profiles. The genetic distance varied from 0.239 to 0.882 indicating a wide range of variation. All 21 rice cultures were categorized into two clusters major clusters comprised of 12 cultures and minor clusters comprised of 9 cultures.