A field experiments were conducted at Agricultural Research Station, Gangavathi, University of Agricultural Sciences, Raichur, Karnataka during kharif seasons for two consecutive years (2019 and 2020) to conduct the effect of zinc and iron biofortification on nutrient availability, uptake and yield of dry direct seeded rice under aerobic condition. The experiment was laid out in split plot design and comprised of two factors for the study viz., main plots and sub plot treatments. Pooled data of two years showed that, among the rice genotypes, G₃: GNV-10-89 recorded significantly higher grain yield (5719 kg ha^-1), straw yield (6825 kg ha^-1), number of panicles (390.03 m^-2), panicle weight (6.42 g), total nitrogen, (121.54 kg ha^-1), phosphorus (42.36 kg ha^-1), potassium (131.75 kg ha^-1), zinc (584.63 g ha^-1) and iron (2485.2 g ha^-1) uptake by the plant. The rice genotype, G₁: RP Bio-226 resulted in significantly higher available nitrogen (249.82 kg ha^-1), phosphorus, (25.10 kg ha^-1), potassium (287.17 kg ha^-1), zinc (0.63 g ha^-1) and iron (4.67 g ha^-1) in the soil as compared to other genotype. With respect to micronutrient application, M₆: Soil application of ZnSO₄ @ 15 kg ha^-1 and FeSO₄ @ 10 kg ha^-1 + foliar application of ZnSO₄ @ 0.5 % and FeSO₄ @ 0.5% at 30 and 45 DAS recorded significantly higher grain yield (5774 kg ha^-1), straw yield (6792 kg ha^-1), number of panicles (391.25 m^-2), panicle weight (6.64 g), total nitrogen (132.68 kg ha^-1), phosphorus (47.03 kg ha^-1), potassium (145.21 kg ha^-1), zinc (692.30 g ha^-1) and iron (2797.5 g ha^-1) uptake by the plant. The micronutrient application, M₆: Control resulted in significantly higher available nitrogen, phosphorus, potassium in soil (249.82, 25.10 and 287.17 kg ha^-1), available zinc and iron in the soil (0.66 and 4.68 ppm) as compared to other micronutrient application. Similar trend was observed during 2019 and 2020.