Vol. 10, Issue 1, Part E (2026)

Present study was undertaken to establish the reference range of thyroid hormones viz. Total triiodothyronine (TT₃), Total thyroxine (TT_4,) and free Thyroxine (fT₄) in Osmanabadi bucks. Total 226 blood samples were collected from healthy adult Osmanabadi bucks from Advy Chemicals Pvt. Ltd. farm located at Phalegaon, Kalyan, Dist. Thane (India). Serum samples were analyzed for thyroid hormones using radioimmunoassay (RIA). Reference ranges for hormone concentrations were established by the non-parametric method of percentile estimates (25th to 75th percentile). The mean value of T₃ of Osmanabadi bucks was 103.15±2.54ng/dl. The box plot of TT₃ concentration in goats where, ‘T bars' represent the range from 19.5 to 201ng/dl, interquartile range (25^th to 75th percentile), as 76.6 to 127.1ng/dl and median 97.68ng/dl. The mean value of TT₄ of healthy Osmanabadi bucks was recorded as 5.43±0.15µg/dl. The box plot of TT₄ concentration in goats where 'T bars' represent the range from 1.18 to 11.2µg/dl, interquartile range (25th to 75th percentile), as 3.8 to 6.9 µg/dl and median as 4.9µg/dl. The mean value of fT₄ of healthy Osmanabadi bucks was recorded as 0.99±0.01ng/dl. The box plot of fT₄ concentration in goats where 'T bars' represent the range from 0.46 to 1.58ng/dl, interquartile range (25th to 75th percentile), as 0.85 to 1.84ng/dl and median as 1.0ng/dl. The established reference range for blood serum levels of TT₃, TT₄ & fT₄ are 76.6 to 127.1ng/dl, 1.18 to 11.2µg/dl and 0.85 to 1.84ng/dl respectively in Osmanabadi bucks. The physiological serum thyroid hormones reference range levels would be valuable information for identifying thyroid dysfunction in Osmanabadi bucks.