Vol. 10, Issue 1, Part B (2026)

Mastitis is a multi-aetiological disease that causes production losses in goats and is characterized by physical, chemical, pathological, and microbiological alterations in milk and glandular tissue. Total 76 bacterial isolates from mastitic milk were identified based on morphological, cultural, and biochemical characteristics. Staphylococcus spp. (39.47%) was the predominant organism (having 33.33% of S. aureus and 66.67% of CNS). By using PCR, 33.33% (10/30) of the culturally positive Staphylococci isolates were identified as S. aureus. Out of 30 isolates of Staphylococcus spp., 80% (24) isolates exhibited biofilm formation by the CRA method. All S. aureus (100%) isolates and 70% (14) CNS isolates exhibited biofilm formation by the CRA method.