Vol. 10, Issue 1, Part B (2026)

The Production and reproduction in dairy cows influence the serum biochemical parameters and the difference in serum biochemical parameters of primiparous and mutliparous animals is influenced by negative energy balance, plane of nutrition, metabolic stress and adaptation to the conditions by the individual animals. Serum biochemical parameters namely Glucose, Albumin, Globulin, Total Protein, Albumin : Globulin, Triglycerides, Total Cholesterol, Creatinine, Aspartate Amino Transferase, Calcium, Magnesium and Phosphorus and antioxidant parameters namely Reduced Glutathione and Super Oxide Dismutase levels were analyzed in Non-Pregnant lactating cows and Dry Pregnant cows in second and third parity cross bred HF Cows. Serum glucose, Magnesium levels were elevated and Total protein level was decreased in Lactating third parity cows compared to dry pregnant cows of third parity. Triacyclglcerol level was elevated significantly in dry pregnant cows compared to lactating cows of both parity. No significant difference was observed statistically in Serum albumin, Globulin, Cholesterol, Creatinine and AST activities between the groups. Reduced Glutathione was elevated in Pregnant cows compared to lactating cows in both parity, while elevation of Superoxide Dismutase was evident in third parity cows alone. Hence in the current study significant changes in serum biochemical parameters and antioxidant levels were observed in certain parameters namely Glucose, Total Protein, Magnesium and Super Oxide Dismutase in third parity cows of different physiological states but not in second parity.