Vol. 10, Issue 1, Part A (2026)

Pyometra in dogs is a life-threatening uterine condition affecting intact bitches. It most commonly occurs during the dioestrous phase of the oestrous cycle. This study focused on the role of Progesterone receptors (PR) in influencing the cervical patency in dogs diagnosed with pyometra. A total of twelve pyometric bitches—six with an open cervix and six with a closed cervix—were included, along with six healthy bitches in dioestrus serving as controls. All animals underwent ovariohysterectomy under a standardized anaesthetic protocol. Cervical tissue samples were collected and stored in RNA later for subsequent gene expression studies. The levels of PR mRNA expression were evaluated using real-time quantitative PCR (qRT-PCR). No statistically significant variation in PR expression was observed among the groups. The findings of the study indicated that PR does not play a role in the regulation of cervical patency.