Vol. 10, Issue 1, Part A (2026)

The present study was carried out to check the ameliorative effect of pomegranate peel extract (PPE) against fipronil-induced hepato-renal toxicity in male Wistar albino rats. A study was conducted for 45 days period in 4 groups of male Wistar albino rats with 6 animals in each group. Group I and III served as vehicle (distilled water) control and Punica granatum control. Group II animals were treated with fipronil @ 10 mg/kg b. wt. orally with distilled water. Group IV animals were treated with both fipronil and pomegranate peel extract @ 10 and 200 mg/kg b. wt. respectively for a period of 45 days using distilled water as a vehicle. Tissue pieces of liver and kidney were subjected for oxidative stress estimation, which revealed a significant increase (P<0.05) in the levels of lipid peroxidation (LPO) and decreased levels of superoxide dismutase, catalase, reduced glutathione and glutathione peroxidase in fipronil-treated rats. Grossly, the liver of fipronil-treated rats was congested and enlarged compared to other groups. Histopathological examination of liver sections of rats in the same group revealed moderate to severe degenerative changes in hepatocytes with loss of architecture, hepatic cords disruption, mononuclear cell infiltration, fatty changes and bile duct epithelial hyperplasia. In kidney section, severe degenerative changes were observed. Co-administration of Punica granatum (pomegranate peel extract) along with fipronil resulted in restoration of liver and kidney enzymes values and oxidative stress parameters near to normal ranges. Histologically, the architectural details of the liver and kidney were regained with minimal degenerative changes. The findings obtained through serum biochemical, oxidative stress estimation, gross and histopathological evaluation clearly revealed the ameliorative effects of Punica granatum (pomegranate peel extract) against fipronil-induced hepato-renal toxicity in male Wistar albino rats.