Vol. 9, Issue 9, Part D (2025)

The brooders of size varied from 375 to 540 g were taken for the present investigation. The Female Koi carp received WOVA-FH at 0.2 ml/kg (T₁), 0.3 ml/kg (T₂), and 0.4 ml/kg (T₃) in triplicate treatments. Similarly male brooders were injected with 0.1 ml/kg (T₁), 0.2 ml/kg (T₂), and 0.3 ml/kg (T₃) WOVA-FH, also in triplicates. Brooders were introduced into breeding hapas at a 1:1 male to female ratio. Brooders treated with 0.3 ml/kg body weight (T₂) showed 100% spawning success, releasing 11814.0±1220.7 no of eggs. In comparison, brooders given 0.2 ml/kg (T₁) released 7870.0±1464.9 eggs, while those treated with 0.4 ml/kg (T₃) laid 9645.00±1019.47 no of eggs. When brooders received a lower dose of 0.2 ml/kg (T₁), only partial spawning occurred. Egg numbers ranged from 6890 to 9554, with fertilization percentages of 65.0±0.7 and hatching rate of 59.29±0.3. Brooders treated with WOVA-FH at 0.3 ml/kg (T₂) showed fertilization and hatching rates of 71.5±0.5% and 64.8±0.1%, respectively. In T₃, the total number of eggs laid ranged from 8741 to 10750, with fertilization rates of 68.31±0.71. Corresponding hatching rates was 62.2±0.4. The findings indicate that WOVA-FH is an effective inducing agent for achieving successful breeding in Koi carp. Administering WOVA-FH at a dosage of 0.3 ml/kg body weight yielded complete breeding success, including the highest egg output along with improved fertilization and hatching rates.