Vol. 9, Issue 8, Part J (2025)

This study examined the effect of incorporating deoiled palm kernel cake (DPKC) into the concentrate ration on the blood biochemical profile and serum cortisol levels of early‑lactation Murrah buffaloes. Twelve multiparous buffaloes (average body weight: 586.58±12.36 kg) in the first two months of their second lactation were equally divided into two groups: a control diet without DPKC (T₁) and a treatment diet where DPKC replaced 15% of the concentrate mixture (T₂). Both rations, provided with ad libitum green fodder, were formulated to meet ICAR (2013) nutrient requirements.

No significant differences (p>0.05) were observed between groups in 6% fat‑corrected milk (FCM) yield. Serum total protein, albumin, globulin, albumin‑to‑globulin ratio, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, glucose, total cholesterol, HDL and LDL cholesterol, triglycerides, urea, creatinine, and cortisol remained within normal physiological ranges, with no treatment effects (p>0.05).

These results demonstrate that inclusion of DPKC at a 15% level does not adversely affect metabolic health, hepatic and renal function, lipid status, or stress hormone levels in lactating Murrah buffaloes. Given its neutral impact on biochemical and hormonal parameters as well as milk productivity, DPKC can be effectively used as a cost‑reducing alternative feed ingredient in concentrate mixtures for early‑lactation buffaloes without compromising health or performance.