Vol. 9, Issue 8, Part I (2025)
Extraction, separation and identification of active compounds in Rosemarinus officinalis by using thin layer chromatographic (TLC) and gas chromatography and mass spectrometry studies
Mavilashaw VP, Saranya M, Kalpana R and Sabarish M
Sohxlet apparatus is used to prepare the plant extract from the leaves of Rosemarinus officinalis by using the solvent of hexane. TLC with hexane solvent of R. officinalis gave 5 spots, and the active compounds were isolated. R. officinalis spots was eluted through TLC employed in GCMS found the presence of 22 active compounds like Eucalyptol, Borneol, Camphor, trans-á-Terpinyl pentanoate, D-Verbenone, Bornyl acetate, Caryophyllene, à-Caryophyllene, Naphthalene, Cedrene, Copaene, trans-Z-à-Bisabolene epoxide, 1-Heptatriacotanol, n-Hexadecanoic acid, Heptamethyl-3-phenyl-1,4-cyclohexadiene, Trenbolone Acetate, 9,12-Octadecadienoyl chloride, (Z,Z)-, and other few unidentified compounds were identified.
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