Vol. 9, Issue 8, Part F (2025)

DNA markers can provide an ideal al ternative method for evaluating genetic diversity in soybean germplasm. Soybean crop is nutritionally important legumes globally. The present investigation was carried out to study molecular and biochemical characterization of soybean (Glycin Max L) by using RAPD markers. PCR amplification was conducted using RAPD primers, specifically OPN-06, OPN-07, OPN-08, OPN-09 and OPN-10. The dendrogram revealed polymorphism at a high percentage. The findings indicated the highest genetic similarity (coefficient = 0.82) between AVT 23-89 and AVT23-92, while the lowest similarity 0.14 was observed between Phule Kimaya and Phule Kalyani in RAPD marker analysis. The genetic diversity uncovered in this study aligns with previous reports by Tidkeet al. (2018), which recorded a polymorphism percentage of 86.95%.