Vol. 9, Issue 8, Part B (2025)

A comprehensive study was conducted to investigate the underlying causes of cervico vaginal prolapse in buffaloes, with a comparative analysis of 10 healthy pregnant buffaloes and 10 buffaloes affected by the condition. The research revealed that the affected animals exhibited no significant difference in Haemoglobin, Packed Cell Volume and DLC, however significantly lower levels of essential minerals was identified between Group I and Group II buffaloes. The results were shown as 10.34±0.45 mg/dl, 6.0±0.19 mg/dl and 164.36±5.0 µg/dl of calcium, phosphorus and zinc respectively in Group-Ⅰ buffaloes and in Group-Ⅱ buffaloes the calcium, phosphorus and zinc levels were found to be 8.33±0.19, 4.89±0.32 and 138.43±5.41 respectively and no significant difference was found in iron levels between Group-I (321.86±9.80 µg/dl) and Group-II (315.29±8.43 µg/dl) buffaloes. Furthermore, the study evaluated oxidative stress status and found non-significant differences in their levels between the healthy and affected buffaloes. The levels of MDA, SOD and Catalase were noted as 0.92±0.09 µ mol/l, 182.39±6.46 u/ml and 4.14±0.41 K units/litre respectively in Group-Ⅰ buffaloes and in Group-Ⅱ buffaloes it was noted as 1.31±0.13 µ mol/l, 116.19±6.5 u/ml and 2.41±0.28 K units/litre levels of MDA, SOD and Catalase respectively. The investigation also explored the role of estrogen hormone in the development of cervicovaginal prolapse, observing non-significant variations in its levels between Group-Ⅰ (52.41±5.78 pg/ml) and Group-Ⅱ (88.27±14.90 pg/ml) buffaloes. This study highlights the need for targeted supplementation, better management, and preventive measures to address mineral deficiencies, oxidative stress, and elevated estrogen levels.