Vol. 9, Issue 4, Part B (2025)

In paper pulp industry it is needed to separate lignin from the cellulose for making white paper. Removal of lignin from wood involves use of different chemicals like sodium hydroxide (NaOH) and sodium sulfide (Na₂S) to remove noncellulose components and leaving the cellulose fibers intact. Thus chemical pulping involves lots of harsh chemical, which creates lots of water pollution and bio-pulping can be sustainably used to mitigate this issue.

In Bio- pulping is the process ligninolytic fungal enzymes (MnP, LiP, AAO, Lacasse) are used to break down lignin. Present work was aimed to analyse and characterize the lignocellulolytic enzyme activities of two white rot fungi Daedaleopsis confragosa and Phellinus pectinatus. D. Confragosa produces ligninolytic enzymes like MnP, LiP, AAO and Laccase, P. pectinatus did not produce MnP and LiP, but produced AAO and Laccase. All the enzymes were stable at pH 5 and active till 45°C temperature. Results of the enzyme activity and stability to different pH and temperature, predicts the optimal conditions which would be suitable for its practical application in biodegradation processes, especially in pulp and paper industries for bio pulping.