Vol. 9, Issue 12, Part J (2025)

 This experiment was conducted at the poultry research farms of the College of Agriculture, Department of Animal Production, Al-Qasim Green University, from November 1, 2024, to January 28, 2025, to determine the effect of an alcoholic extract of powdered graviola (Annona muricata) leaves on sex hormones (testosterone and estrogen) and antioxidant enzymes in Ross 308 broiler chickens. Sixteen Ross 308 roosters were used and randomly assigned to four experimental groups, with four replicates per group (one rooster per replicate). The experimental groups were as follows: Group 1 (T₁)-control group (no supplement); Group 2 (T₂)-alcoholic extract of powdered graviola (Annona muricata) leaves added to drinking water at a concentration of 10 ml/L; Group 3 (T₃)-15 ml/L; and Group 4 (T₄)-20 ml/L. The main objective was to evaluate the effect of the alcoholic extract of the graviola plant (Annona muricata) on hormone and enzyme properties. The extract was prepared in the laboratories of the College of Science/University of Babylon according to the methods described in the Materials and Methods section. The results showed that treatments T₄ and T₃ significantly improved sex hormone levels, with treatments T₄ and T₃ showing an increase in testosterone and a decrease in estrogen in male broiler breeder hens. The fourth treatment also recorded a clear improvement in the concentration of the glutathione enzyme (GSH) compared to the control treatment, and a decrease in the concentration of the MDA enzyme in the fourth treatment compared to the control treatment.