Vol. 9, Issue 11, Part F (2025)

A present study was carried out from August 2023 to July 2024 on 14 dogs suffering from periodontal disease to evaluate the use of dental scaling with ozonated water and demineralized water (7 dogs each). Bacterial culture and isolation revealed Gram-positive cocci as the predominant organisms, with Amikacin demonstrating the highest level of antimicrobial sensitivity among the tested antibiotics. Following dental scaling, a marked reduction in bacterial load (CFU/mL) was observed in the ozonated water group, decreasing from 74.14±8.13 to 32.57±4.77 × 10³ CFU/mL. In contrast, the demineralized water group showed only a minor decline, from 79.86±8.43 to 70.00±7.61 × 10³ CFU/mL. These results highlight the superior antibacterial efficacy of ozonated water in reducing oral microbial burden during periodontal therapy. This is likely due to the antibacterial properties of ozonated water, which are attributed to its oxidative nature. These findings indicate that ozonated water is more effective in reducing bacterial load, demonstrating superior efficacy in the management of canine periodontal disease.