Vol. 9, Issue 11, Part C (2025)

Background: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the world. In particular, the transmembrane 6 superfamily member 2 (TM6SF2) gene's rs58542926 (C>T) SNP has been connected to lipid metabolism and liver fat accumulation. The connection between the TM6SF2 rs58542926 polymorphism and some biochemical markers such as lipids profile and liver functions were investigated in patients with NAFLD from Iraq. Methods: A case-control study comprising of 60 NAFLD patients and 40 healthy controls. Anthropometric, biochemical, and lipid parameters were measured. TM6SF2 rs58542926 SNP and genotype analysis were detected by polymerase chain reaction-restriction fragment length polymorphism (PC-RFLP). Standard t-test, Chi-square and ANOVA, with p-value less than 0.05 considered significant. Results: This study suggested that BMI, ALT, AST, TC, TG and LDL-C were found a higher levels while HDL-C was found lower levels in NAFLD patients (all p<0.001). Genotype distribution in NAFLD group were (CC: 33.3%, CT: 46.7%, TT: 20.0%) while in controls (CC: 62.5%, CT: 30.0%, TT: 7.5%) and this results shows statistically significant (p = 0.012). TG, LDL-C levels were lower in T allele carriers (p<0.05). Conclusion: The TM6SF2 rs58542926 T allele is significantly associated with NAFLD in Iraqi patients and correlates with reduced serum TG and LDL-C. This study suggests that TM6SF2 rs58542926 SNP as a genetic risk factor of hepatic lipid metabolism and a potential marker for NAFLD risk assessment.